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KMID : 0545119910010010045
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Journal of Microbiology and Biotechnology
1991 Volume.1 No. 1 p.45 ~ p.49
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The Complete Nucleotide Sequence of Alkalophilic Bacillus sp. K-17 β-Xylosidase Gene
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Chun, Hyo Kon
Ko, Hak Ryong/Kho, Yung Hee/Sung, Nack Kie
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Abstract
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The complete nucleotide sequence of alkalophilic Bacillus sp. K-17 β-xylosidase gene and its flanking regions were established. A 1263-bp of an open reading frame for β-xylosidase was observed. The molecular weight (50, 521 dalton), deduced from the nucleotide sequence of β-xylosidase gene, agreed with the result obtained by SDS-polyacrylamide gel electrophoresis of the purified enzyme (51,000 dalton). The Shine-Dalgarno sequence, 5´-GAGGAGG-3´, was found 8 bp upstream of the initiation codon ATG. The -10 sequence (TAAAAT) in the promoter region for β-xylosidase gene was similar to the consensus sequence for Bacillus subtilis RNA polymerase, whereas the -35 sequence (TCGATCA) different from all the known -35 regions in the promoter for Bacillus subtilis RNA polymerase.
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KEYWORD
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